In this hybrid lecture and laboratory course students will learn the theory and practice of genetic engineering, particularly applications to advancing discovery in the biotechnology industry. The course will cover transfer of genetic material to host organisms and modification of the hosts themselves to achieve targeted metabolic and cellular outcomes. Topics include the practical assembly of genes, gene circuits, and genomes, from classical methods like PCR and restriction enzyme cloning to modern methods like CRISPR-Cas9 editing and DNA synthesis. Lectures may also cover associated topics like recombination, primer design, combinatorial and mutagenic library assembly, and the utilities of various established and novel host cell systems.
In the laboratory, students will employ individualized design strategies to affect a genetic modification using techniques like CRISPR that will yield a specified biochemical outcome.
Students may not receive credit for both BB 576 and BB570-ST Genetic Engineering and Expression Systems or CH/BB 4170.
Prerequisites/Recommended Background/Prerequisites: No prerequisite courses. Familiarity with underlying concepts in molecular biology, genetics, and cell biology as covered in BB 575 Advanced Genetics and Cellular Biology or BB 504 Molecular Biology of the Cell is recommended.
Learning Outcomes:
Upon completion of this course, students will be able to…
• Design a multi-step process by visualizing the connections between and weighing the influences of gene editing techniques and regulatory framework on workflow
• Design and deliver essential elements of CRISPR (i.e., Cas9 and guide RNA) to alter expression of a target gene
• Connect course outcomes and apply knowledge gained in the course to chosen field of study/career path
• Communicate results, analyses, successes, and challenges appropriately to a defined audience
• Function effectively as a member of an interdisciplinary and diverse team of scientists and engineers
Familiarity with underlying concepts in molecular biology, genetics, and cell biology as covered in BB 575 Advanced Genetics and Cellular Biology or BB 504 Molecular Biology of the Cell is recommended.